Evaluation of Water Quality of the Karun River in Ahvaz, Iran Based on Biological Indicators (Daphnia and Crustaceans)

Abdolkazem Neisi, Mana Ghanbari, Vafa Hamid, Seyedeh Leila Dehghani, Majid Farhadi


Given the fact that the Karun River, as the most important source of water supply in Khuzestan province, has recently been affected by various pollutants such as sewage, monitoring its water quality is a necessity. This study aimed to determine the water quality of the Karun River based on biological indicators (Daphnia-crustaceans).
In each season of the year, water samples were obtained from three stations at three different points along the river. After measuring the physical and chemical parameters, the samples were left to settle for 24 hours in the laboratory. Then 1 cc of the sediment was placed on the slide to study the diversity and frequency of crustaceans. Data were analyzed using SPSS version 21.
In the present study, the frequency and variety of crustacean species isolated from the samples decreased from the initial station of the river to the final station. In addition, 27 species of Protozoa were isolated from three stations, of which Daphnia and Rotifer species with an average of 2.166 and 4.833 and standard deviation of 1.984 and 5.166, respectively had the highest frequency compared to other species. The results of the Pearson correlation showed that there is a direct and statistically significant relationship between dissolved oxygen (DO), Turbidity, Daphnia, and Rotifers.
Based on the results of this study, it can be stated that the entry of effluents into river water causes a significant reduction in water quality, which in turn reduces the diversity and frequency of crustaceans living in the water. This is possibly due to an increase in sewage discharge from Ahvaz city to Karun river.


Biological Indicators, Water Quality, Crustaceans, Daphnia, the Karun River, Iran

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Allan R, Förstner U, Salomons W. Sediment Dynamics and Pollutant Mobility in Rivers An Interdisciplinary Approach (Environmental Science and Engineering) 2007th Edition, Kindle Edition.

Sharma K and Chowdhary S .Macroinvertebrate assemblages as biological indicators of pollution in a Central Himalayan River, Tawi (JK). Int J Biodivers Conserv . 2011;3(5):167-74 .

Rostami S and Qishlaqi A.Contamination and fractionation of heavy metals in bedload sediments of the Siahrood River (Qaem-Shar area-Mazandaran Province) Journal of Stratigraphy and Sedimentology Researches.2016; 32(63):73-90 .

Glenn EP, Flessa KW,Pitt J . Restoration potential of the aquatic ecosystems of the Colorado River Delta, Mexico: introduction to special issue on “Wetlands of the Colorado River Delta” Ecological Engineering.2013; 59:1-6.

Gresens S, Smith R, Sutton-Grier A,Kenney M (2009) Benthic macroinvertebrates as indicators of water quality: The intersection of science and policy Terr Arthropod Rev.2009;2:99-128 .

Jalili S Water Quality Assessment Based on HFB I& BMWP Index In Karoon River, Khouzestan Provience,(Northwest of Persian Gulf) Anthropogenic Pollution Journal.2020; 4(6):36-49 .

Carter JL, Resh VH,Hannaford MJ .Macroinvertebrates as biotic indicators of environmental quality. In: Methods in stream ecology. Elsevier.2017 . 293-18 .

Aura CM, Raburu PO,Herrmann J . A preliminary macroinvertebrate Index of Biotic Integrity for bioassessment of the Kipkaren and Sosiani Rivers, Nzoia River basin, Kenya. Lakes & Reservoirs: Research & Management .2010;15:119-28 .

Mul C, Raburu PO,Herrmann J . Macroinvertebrates community structure in Rivers Kipkaren and Sosiani, River Nzoia basin. Kenya J Ecol Natural Environ2011; 3:39-46.

Redondo-Hasselerharm PE, Falahudin D, Peeters ET,Koelmans AA. Microplastic effect thresholds for freshwater benthic macroinvertebrates. Environmental science & technology.2018;52:2278-86.

Padhye SM,Dahanukar N. Distribution and assemblages of large branchiopods (Crustacea: Branchiopoda) of northern Western Ghats. India Journal of Limnology.2015. 74:371-80.

Neisi A, Maleki H, Hamid V, Vosoughi M,Moradi P. Identifying and counting zooplanktons and crustaceans in water of Karun River, Ahvaz city, Iran Sustainable Water Resources Management.2019; 5:1929-38.

Rosal R, Rodea-Palomares I, Boltes K, Fernández-Piñas F, Leganés F, Gonzalo S,Petre A . Ecotoxicity assessment of lipid regulators in water and biologically treated wastewater using three aquatic organisms. Environmental Science and Pollution Research.2010; 17:135-44.

Siciliano A, Gesuele R, Pagano G,Guida M. How Daphnia (Cladocera) assays may be used as bioindicators of health effects? Journal of Biodiversity & Endangered Species. 2015;1(5):1-6.

Das P, Xenopoulos MA,Metcalfe CD. Toxicity of silver and titanium dioxide nanoparticle suspensions to the aquatic invertebrate, Daphnia magna Bull Environ Contam Toxicol. 2013; 91:76-82

Piscia R, Colombini M, Ponti B, Bettinetti R, Monticelli D, Rossi V,Manca M. Lifetime response of contemporary versus resurrected Daphnia galeata Sars (Crustacea, Cladocera) to Cu(II) chronic exposure. Bull Environ Contam Toxico. 2015;l 94:46-51.

Atashbar B . Effects of physicochemical parameters of water on seasonal variation, distribution and density of crustacean zooplankton communities in Sulduz Wetland (Southern parts of Urmia Lake). Iranian Scientific Fisheries Journal. 2018;26:81-92.

Madadinia M, Monavari M, Karbasi A, Nabavi MB,Rajabzade E . Study on water quality of Karoun river (Ahvaz region) using water quality index . Journal of Environmental Science and Technology.2014;16:49-60.

Peyghan S, Savari A, Sakhaee N, Doustshenas B,Dehghan-Madiseh S. A Study on identification and density of Cyclopoida and Harpacticoida (Crustacea: Copepoda) in Bahrakan water, Handijan Harbor (NW Persian Gulf).Iran Journal of Animal Research.2014; 27:319-28.

Sabkara J,Makaremi M . The density and distribution of the plankton, and their role in fish culture in Arasreservoir dam. Journal of Aquaculture Development.2013. 7:41-59 .

Salavatian S, Abdolahpour Biria H, Nezami Balouchi S, Makaremi M,Pourgholami M. Identification and comparison of seasonal phytoplankton density in Lar dam lake. Journal of Wetland Ecobiology.2010; 2:26-38.

Vosoughi A, Hosseinzadeh Sahafi H,Rajabi A. Identification and diversity assessment of crustacea of Mongoft Artificial Reefs in Bushehr Province, Persian Gulf. Journal of Marine Science and Technology Research.2010;19:13-28.

Seif A,Najmi N . Detecting changes of meanders in Karun river using multi-temporal images of IRS and Landsat .Geographical Researches.2013; 28:211-26.

Ehteshami M, Biglarijoo N,Salari M. Assessment and quality classification of water in Karun, Dez and Karkheh Rivers. Journal of River Engineering .2014;2:23-30.

Yousefi S, Pourghasemi HR, Hooke J, Navratil O,Kidová A. Changes in morphometric meander parameters identified on the Karoon River, Iran, using remote sensing data. Geomorphology. 2016;271:55-64.

Neisi A, Vosoughi M, Mousavian G, Shokouhi E, Moradi M,Motavalian A. Identifying and Counting Prevalent Algae in Karun River in Ahvaz City. Journal of Environmental and Occupational Health.2016; 2:191-00.

Neisi A, Mohammadi MJ, Takdastan A, Babaei AA, Yari AR,Farhadi M. Assessment of tetracycline antibiotic removal from hospital wastewater by extended aeration activated sludge. Desalination and water treatment.2017: 80:380-86.

Varnosfaderany MN, Ebrahimi E, Mirghaffary N,Safyanian A. Biological assessment of the Zayandeh Rud River, Iran, using benthic macroinvertebrates. Limnologica .2010;40(3):226-32.

Cristale J, Vázquez AG, Barata C,Lacorte S. Priority and emerging flame retardants in rivers: occurrence in water and sediment, Daphnia magna toxicity and risk assessment. Environment international.2013;59:232-43.

Iranian Journal of Health, Safety and Environment e-ISSN: :2345-5535 Iran university of Medical sciences, Tehran, Iran