Significance of authenticity in meat and meat products in Iran

Tahereh Rezazadeh, Khosrow Aghaiypour, Zohreh Heidari

Abstract


The Authenticity of meat products is very important for religious and health reasons in Iran. According to legislation in Iran, the consumption and importation of pork, horse, donkey and cat products should be banned. Therefore, the identification of meat products cannot be judged solely by its appearance. This issue led to the authenticity of bovine, sheep, pig, horse, donkey, chicken and soya (Glycine max) in raw and processed meat products.In this study, specific primers were designed for the identification of pig ( base pair), donkey (325 base pair), chicken (391 base pair), sheep (499 base pair), horse (607 base pair), soya (707 base pair) and bovine (853 base pair) by Polymerase chain reaction. Following PCR, expected,, , 499,,  and  base pair fragments were detectable in pig, donkey, chicken, sheep, horse, soya and bovine, respectively. This protocol can be used for identification of raw and processed meat products in various animal species for replication to regulatory obligations for meat species safety in Iran.

Keywords


Authenticity; PCR; Meat products; Meat safety; Iran

Full Text:

PDF

References


Doosti A, Ghasemi Dehkordi P, Rahimi E. Molecular assay to fraud identification of meat products. J Food Sci Technol. ; 51(1): 148-52.

Unajak S, Meesawat P, Anyamaneeratch K, Anuwareepong D, Srikulnath K, Choowongkomon K. Identification of species (meat and blood samples) using nested-PCR analysis of mitochondrial DNA. Afr J Biotechnol. ;  (29): -.

Hsien-Chi W, Shu-Hwae L, Tien-Jye C, Min-Liang W. Examination of meat components in commercial Dog and Cat feed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR- RFLPs) technique. J Vet Med Sci. 4; 66(7): 855-59.

Hsieh YHP, Chen FC, Sheu SC. AAES research developing simple, inexpensive tests for meat products. Highlights of agricultural research. 1997; 44 (2):19-20.

Country brief on IRAN [http://www.tdap.gov.pk/doc_reports/TDAP%20REPORT%20ON%20IRAN.pdf]

Iran to import meat from New Zealand [http://news.xinhuanet.com/english/world/2013 07/18/c_132553296.htm]

Ayaz Y, Ayaz N D, Erol I. Detection of species in meat and meat products using Enzyme‐Linked Immunosorbent Assay. J Muscle Foods. 2006; 17 (2): 214-20.

Asensio L, González I, García T, Martin R. Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA). Food Control. 2008; 19 (1): 1-8.

ELISA and PCR method allergen testing in food products [http://www.microbac.com/uploads/Technical%20Articles/pdf/ELISA%20and%20PCR%20Method%20Allergen%20Testing%20in%20Food%20Products.pdf]

Spychaj A, Mozdziak PE. Pospiech E. PCR methods in meat species identification as a tool for the verification of regional and traditional meat products. Acta Sci Pol Technol Aliment. 2009; 8 (2): 5-20.

Abdel-Rahman SM, El-Saadani MA, Ashry KM, Haggag AS. Detection of adulteration and identification of Cat’s, Dog’s, Donkey’s and Horse’s meat using species-specific PCR and PCR-RFLP techniques. Aust J Basic Appl Sci. ;  (3): -.

Aslan Ö, Hamill RM, Sweeney T, Reardon W, Mullen AM. Integrity of nuclear genomic deoxyribonucleic acid in cooked meat: Implications for food traceability. J Anim Sci. ;  (1): -.

Ballin NZ, Vogensen FK, Karlsson AH. Species determination – Can we detect and quantify meat adulteration. Meat Sci. ;  (2): -.

Chen SY, Liu YP, Yao YG. Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial S rRNA gene. J Genet Genomics. ;  (11): -.

Corona B, Lleonard R, Carpio Y, Uffo O, Martínez S. Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program Span. J Agric Res. ;  (3): -.

Fumière O, Veys P, Boix A, Holst CV, Ilhak OI, Arslan A. Identification of meat species by Polymerase Chain Reaction (PCR) technique. Turk J Vet Anim Sci. ;  (3): -.

Ghovvati S, Nassiri MR, Mirhoseini SZ, Heravi Moussavi A, Javadmanesh A. Fraud identification in industrial meat products by multiplex PCR assay. Food Control. ;  (8): -.

Gupta R, Rank DN, Joshi CG. Duplex-PCR for identification and differentiation of cattle and buffalo processed meat. J Adv Vet Res. 2;  (1): -.

Lopez-Calleja I, Gonzalez I, Fajardo V, Martin I, Hernandez PE, Garcia T, Martin R. Application of polymerase chain reaction to detect adulteration of Sheep’s milk with Goats’ milk. J Dairy Sci. ;  (9): -.

Mafra I, Ferreira IMPLVO, Oliveira MBPP: Food authentication by PCR-based methods. Eur Food Res Technol. ;  (3): -.

Mane BG, Mendiratta SK, Tiwari AK. Polymerase chain reaction assay for identification of chicken in meat and meat products. Food Chem. ;  (3): -.

Montowska M, Pospiech E. Authenticity Determination of meat and meat products on the protein and DNA basis. Food Rev Int. 0;  (1): -.

Spychaj A, Mozdziak PE, Pospiech E. PCR methods in meat species identification as a tool for the verification of regional and traditional meat products. Acta Sci Pol Technol Aliment. ;  (2): -.

Tanabe S, Hase M, Yano T, Sato M. A real-time quantitative PCR detection method for Pork, Chicken, Beef, Mutton, and Horseflesh in foods. Biosci Biotechnol Biochem. ;  (12): -.

High Pure PCR Template Preparation Kit [https://cssportal.roche.com/LFR_PublicDocs/ras/11796828001_en_16.pdf]

Schlegel M, Ali HS, Stieger N, Groschup MH, Wolf R, Ulrich RG. Molecular identification of small mammal species using novel cytochrome B gene-derived degenerated primers. Biochem genet. 2012; 50 (5-6): 440-47.

Hsieh HM, Tsai CC, Tsai LC, Chiang HL, Huang NE, Rocky T.P.S, Linacre A, Lee JC. Species identification of meat products using the cytochrome b gene. Forensic Sci J. 2005; 4: 29-36.

Zhou X, Liu W, Lian J, Zhang WQ. Monitoring of Roundup™ Ready soybean in Guangdong province in China. Food control. 2007; 18 (10): 1219-22.

Xu X, Gullberg A, Arnason U. The complete mitochondrial DNA (mtDNA) of the donkey and mtDNA comparisons among four closely related mammalian species-pairs. J Mol Evol. 1996; 43 (5): 438-46.

Porto Neto, LR, Jonsson NN, D’Occhio MJ, Barendse W. Molecular genetic approaches for identifying the basis of variation in resistance to tick infestation in cattle. Vet Parasitol. 2011; 180 (3): 165-72.

Horsemeat found in beefburgers on sale in UK and Ireland [www.bbc.co.uk/news/world-europe-21034942]

Meat Speciation and authenticity [http://www.absciex.com/applications/food-and-beverage-testing/ingredient-analysis/meat-speciation-and-authenticity]




Iranian Journal of Health, Safety and Environment e-ISSN: :2345-5535 Iran university of Medical sciences, Tehran, Iran